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It is responsible for the transfer of phosphate from ATP to Ser or Thr residues in substrates.The autoinhibitory domain features a pseudosubstrate site, which binds to the catalytic domain and blocks its ability to phosphorylate proteins.) and transfers phosphates from ATP to defined serine or threonine residues in other proteins.

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All of the isoforms of Ca MKII have: a catalytic domain, an autoinhibitory domain, a variable segment, and a self-association domain.

The catalytic domain has several binding sites for ATP and other substrate anchor proteins.

Once the Threonine 286 residue has been phosphorylated, the inhibitory domain is blocked from the pseudosubstrate site.

This effectively blocks autoinhibition, allowing for permanent activation of the Ca MKII enzyme.


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